Data/hora: 18/05/2024 23:46:53

Biblioteca(s): 	Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido.	
Data corrente: 	18/09/2020	
Data da última atualização: 	17/11/2020	
Autoria: 	MELO, B. P. de; LOURENCO-TESSUTTI, I. T.; MORGANTE, C. V.; SANTOS, N. C.; PINHEIRO, L. B.; LINS, C. B. de J.; SILVA, M. C. M.; MACEDO, L. L. P.; FONTES, E. P. B.; GROSSI-DE-SA, M. F.	
Afiliação: 	BRUNO PAES DE MELO, UFV; ISABELA TRISTAN LOURENCO TESSUTTI, Cenargen; CAROLINA VIANNA MORGANTE, CPATSA; NAIARA CORDEIRO SANTOS; LUANNA BEZERRA PINHEIRO, UCB; CAMILA BARROZO DE JESUS LINS; MARIA CRISTINA MATTAR DA SILVA, Cenargen; LEONARDO LIMA PEPINO DE MACEDO, Cenargen; ELIZABETH PACHECO BATISTA FONTES, UFV; MARIA FATIMA GROSSI DE SA, Cenargen.	
Título: 	Soybean embryonic axis transformation: combining biolistic and Agrobacterium-Mediated Protocols to overcome typical complications of in vitro plant regeneration.	
Ano de publicação: 	2020	
Fonte/Imprenta: 	Frontiers in Plant Science, v. 11, article 1228, 2020.	
DOI: 	https://doi.org/10.3389/fpls.2020.01228	
Idioma: 	Inglês	
Conteúdo: 	The first successful attempt to generate genetically modified plants expressing a transgene was preformed via T-DNA-based gene transfer employing Agrobacterium tumefaciens-mediated genetic transformation. Limitations over infectivity and in vitro tissue culture led to the development of other DNA delivery systems, such as the biolistic method. Herein, we developed a new one-step protocol for transgenic soybean recovery by combining the two different transformation methods. This protocol comprises the following steps: agrobacterial preparation, seed sterilization, soybean embryo excision, shoot-cell injury by tungsten-microparticle bombardment, A. tumefaciens-mediated transformation, embryo co-cultivation in vitro, and selection of transgenic plants. This protocol can be completed in approximately 30?40 weeks. The average efficiency of producing transgenic soybean germlines using this protocol was 9.84%, similar to other previously described protocols. However, we introduced a more cost-effective, more straightforward and shorter methodology for transgenic plant recovery, which allows co-cultivation and plant regeneration in a single step, decreasing the chances of contamination and making the manipulation easier. Finally, as a hallmark, our protocol does not generate plant chimeras, in contrast to traditional plant regeneration protocols applied in other Agrobacterium-mediated transformation methods. Therefore, this new approach of plant transformation is applicable for studies of gene function and the production of transgenic cultivars carrying different traits for precision-breeding programs.	
Palavras-Chave: 	Agrobacterium-mediated transformation; Embryonic axis; High-efficiency plant transformation; Particle bombardment; Planta geneticamente modificada; Recuperação trangênica de soja.	
Thesagro: 	Cultura de Tecido; Glycine Max; Soja.	
Thesaurus Nal: 	Agrobacterium; Embryonic structures; Genetic transformation; Plant genetics.	
Categoria do assunto: 	--
G Melhoramento Genético	
URL: 	http://ainfo.cnptia.embrapa.br/digital/bitstream/item/216085/1/fpls-11-01228.pdf	
Registro original: 	Embrapa Recursos Genéticos e Biotecnologia (CENARGEN)	
Exemplares: 	CENARGEN - (38269-1) - UPC - AP - DD -  -  -  - DIS
CPATSA - (59240-1) - UPC - AP - DD -  -  -  - DIS